Deoxynucleoside Kinases of Bacillus megaterium KM
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چکیده
منابع مشابه
Masking of Bacillus megaterium KM membrane reduced nicotinamide adenine dinucleotide oxidase and solubilization studies.
Solubilization of a reduced nicotinamide adenine dinucleotide (NADH)-2,6 dichlorophenol indophenol (DCIP) oxidoreductase associated with the membrane NADH oxidase system of Bacillus megaterium KM was effected by treatment with 0.2% sodium deoxycholate, 8 m urea, or buffer (pH 9.0) in the presence of ethyl-enediaminetetraacetate. These treatments inactivated membrane NADH oxidase. It was found t...
متن کاملZinc release and the sequence of biochemical events during triggering of Bacillus megaterium KM spore germination.
Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. Of the total spore Zn2+ pool 25% is released from non-heat-activated spores within 4 min of triggering germination. During this period only 10% of the spore population becomes irreversibly committed to germinate. The investigation of a putative role for Zn2+ in ...
متن کاملPurification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM.
Two peptidoglycan-lytic enzyme activities were isolated from spores of Bacillus megaterium KM. Surface-bound lytic enzyme was extracted from dormant spores and hydrolysed a variety of peptidoglycan substrates including isolated spore cortex, but did not cause refractility changes in permeabilized spores. Germination-specific lytic enzyme activity appeared early in germination and had minimal ac...
متن کاملCharacterization, purification and synthesis of spore-coat protein in Bacillus megaterium KM.
The spore-coat fraction from Bacillus megaterium KM, when prepared by extraction of lysozyme-digested integuments with SDS (sodium dodecyl sulphate) and urea, contains three N-terminal residues and a major component of apparent mol.wt. 17500. Electron microscopy of this fraction shows it to consist of an ordered multilamellar structure similar to that which forms the coat region of intact spore...
متن کاملElectron-Transport Systems Obtained from Vegetative Organisms and Spores of Bacillus megaterium KM
succinate contained increasing amounts of wax as the specific growth rate decreased from 0.7h-I to 0.12h-I. The wax composition also varied with dilution rate, samples having a high wax content had palmitic acid and palmityl alcohol as major components. In continuous culture with succinate limitation and excess of nitrogen, the bacteria contained only small amounts of waxes, which increased sli...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1971
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.105.3.787-792.1971